Group 6 - Del Mundo
CONCLUSION
After collecting and analyzing the data from this study, the researchers conclude the following:​
1. Based on the results of the screening, the guyabano extract exhibited the presence of terpenoids, flavonoids, amino acids, and tannins. These metabolites are associated with the mediation of the nanoparticle biosynthesis, specifically in the reduction of copper ions into CuNPs.
2. There was no significant difference within the different concentrations of CuNPs suggesting that the difference in concentrations has no significantly distinct impact on the mortality rate of the brown dog ticks.
3. There is a significant difference between the experimental group and the control group. This signifies that although the concentrations have a 100% mortality rate, its effectiveness is still lacking behind the positive control group, especially in terms of the speed of how fast it exterminates the experimental units
RECOMMENDATIONS
In the light of the replication of this paper, the researchers suggest the following to further improve the study:
1. Explore other parts of the guyabano plant such as the fruit itself, the roots, or the stem, since most papers already utilize the use of leaves as the source of the extract. Leaves were used in this study as the concept of synthesizing copper nanoparticles using guyabano leaf ethanolic extract is a new concept.
2. Increase the sample size per petri dish of the brown dog ticks to increase the accuracy of generalization. The researchers were only able to acquire two dog ticks per petri dish because of the scarcity of samples and the lack of time to collect.
3. Study if the increased amount of ethanol in less concentrated solutions (e.g. 5%) affects the extermination of the experiment. The ethanol combined with the CuNPs in the experiment was volatile and was prone to evaporation, it would be beneficial to find an alternative solvent that is less volatile and will hold the solution for a much longer period of time.
4. Consider using other plants to mediate the production of nanoparticles and also other metals that have more established properties that could help get rid of brown dog ticks. Other experimental units may also be considered such as the Ctenocephalides felis (Cat fleas) and Sarcoptes scabiei (Sarcoptic mange) which are also common ectoparasites in domestic animals. These would add to the current evidence of effectiveness of natural pesticides and acaricides.
5. Conduct a sessional application of the treatment, instead only applying the treatment once, and observe for a much longer period of time at larger intervals (e.g. every 2 hours for 12 hours) since the ticks tend to have a defense mechanism that make it seem as if they are dead even though they are not.
6. Experiment on the practical applications of the guyabano-mediated CuNPs such as in combination with topical and oral medication to maximize their purpose.
7. Invest in laboratories ran by experts that can guide you throughout the experiment. The laboratory in the school is always busy; thus, schedules are always moved and samples could be affected.